valitatiter assay

Results in minutes not hours.

Valita Titer assay is a plate based, 96- and 384-well assay that offers a rapid, cost effective way to measure IgG. Valita Titer assay is among the industry’s fastest IgG tests and is compatible with all plate readers with a Fluorescence Polarization module.

Straight from culture to plates and results

  • No sample preparation
  • No additional reagents
  • No wash

Measure IgG directly in cell suspension

  • Simple: Add, mix & read
  • Rapid: Results in less than 15 minutes
  • Automation friendly: 3 steps in standard plates
  • Value for money: Competition-beating prices

Rapid & Easy to Use

Valita Titer assay is among the industry’s fastest and easiest IgG quantification assay in the industry and has a number of advantages over other long and tedious techniques like ELISA and HPLC.

valitatiter assay plates

Step 1: Add Sample

Add cell suspension to the plate

valitatiter incubation time

Step 2: Incubate

Incubate for 5 minutes at Room Temperature

microplate reader

Step 3: Measure IgG

Quantify IgG using a plate reader

Total Assay Time < 15 minutes

  • Productivity Clone Ranking
  • Transfection QC for Productivity
  • Clone Expression Optimization
  • Stable Pool Characterization
  • Assay time: Add, Mix, Read < 15 minutes (96-well Valita Titer plates)
  • Productivity Screening
  • Clone Ranking for Scale-up Selection
  • Process Optimization
  • Production Process Productivity Monitoring
  • 30 minutes (96-well Valita Titer Plus plates)

Accurate & Precise Quantification

Valita Titer assay is accurate and precise and results are well correlated to industry standard techniques like Protein A HPLC and BLI.

valitatiter comparison with other techniques 


Valita Titer Assay Product Details

  • Assay accuracy: > 95%
  • Detection range and precision
  • Valita Titer assay: 2.5 mg/L -100 mg/L
  • Valita Titer Plus assay: 100 mg/L - 2000 mg/L
  • Plate format: 96- and 384-well plate formats
  • Assay precision: < 2.5% RSD for inter- and intra-assay precision
  • Cell robustness: Up to 15x10^6 cells/mL
  • Fluorescent label: FITC (Excitation max. = 490, Emission max. = 525)
  • IgG binding peptide: Fc binding peptide: binding domain 1 of Protein G
  • IgG species: Validated for Human and Rabbit
  • IgG formats: All Fc-containing IgGs (standard, bispecific, fusion proteins)
  • IgG subclasses: 1,2,3,4 (human)

© 2023 ValitaCell Ltd. All rights reserved. Valita, Valita Titer and the ValitaCell logo are the trademarks of ValitaCell Ltd in the United States and other countries. ValitaCell is a Beckman Coulter Company. Not intended or validated for use in the diagnosis of disease or other conditions.

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