Cell Line Development and the Valita Titer Assay

Cell line development is the process of establishing and optimizing a stable and reproducible cell line for use in research, biomanufacturing or drug discovery. It involves the selection and isolation of a specific cell type with desired characteristics, followed by the establishment of a clonal cell population that can be grown and expanded.

The Valita Titer assay plays a significant role in cell line development by providing a quantitative measurement of the productivity or expression level of monoclonal antibodies in a given cell line. It’s used to determine the IgG titer produced by the cell line, which is crucial for evaluating the performance and productivity of the selected clone.

Rapid Clone Productivity Screening

In cell line development, being able to accurately and rapidly measure monoclonal antibody titer is critical.

Cell Line Development Workflow with Valita Titer Assay 

The Valita Titer assay can enable cell line development labs to prepare and measure a full 96- or 384-well plate in as little as 15 minutes. It’s among the industry’s fastest IgG tests and is compatible with most automation platforms , including our Biomek i-Series Automated Workstations.

  • Get results in less than 15 minutes
  • Available in 96- and 384-well plate options
  • No sample preparation, additional reagents or wash required

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A Story About Developing Scalable, High-Yielding CHO Cell Lines

Thanks in part to the Valita Titer assay, ExcellGene—a Swiss biologics CDMO—was able to develop scalable, high-yielding (4-8 g/L) Chinese hamster ovary (CHO) cell lines in just eight weeks.

Watch as ExcellGene’s Dr. Joeri Kint explains how being able to measure IgG directly in cell suspension and go straight from culture to plates and results is enabling them to rapidly select high-producing clones.

 


Insightful Resources

Application Note
High-throughput IgG quantitation platform for clone screening during drug discovery and development.

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Application Note
Improved data quality of plate-based IgG quantification using Spark®’s enhanced optics

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Learn More About the Valita Titer Assay

Introducing Valita Titer Assays

Valita Titer plate-based, 96- and 384-well assays that offer a rapid, cost-effective way to measure IgG

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Compare IgG Measurement Technologies

See how the Valita Titer assay compares to Protein A HPLC, ELISA and biolayer interferometry

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Say Goodbye to Sample Preparation

Measure IgG titer straight from cell culture samples—no sample prep, additional reagents or wash required

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Automate With Ease

Easily integrate Valita Titer into your automated liquid handler and microplate reader

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Let’s Accelerate Your Antibody Discovery and Development

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© For Research Use Only. Not for use in diagnostic procedures. Valita, Valita Titer and the ValitaCell logo are the trademarks of ValitaCell Ltd in the United States and other countries. ValitaCell is a Beckman Coulter Company.