Extraction d'ARN à Partir des Cellules

Le kit Agencourt RNAdvance Cell v2 est basé sur la technologie d'immobilisation réversible en phase solide par billes paramagnétiques, connue pour sa capacité à toujours délivrer le plus haut rendement et la meilleure qualité pour les acides nucléiques purs. Ce système assure, avec fiabilité, une récupération et une pureté importantes de l’ARN sans avoir à recourir à la filtration ou la centrifugation. Les éléments chimiques du système Agencourt RNAdvance Cell v2 permettent l'automatisation en matière d'isolation de l’ARN total pour des applications en microarray et en PCR quantitative en temps réel.

Choose a From Cells Model

RNAdvance Cells Workflow Steps

Genomics RNAdvance Cell v2 Workflow

Product Features

Overview
RNAdvance Cell V2 isolates total RNA from cultured cells.

High Quality RNA
The RNAdvance Cell V2 isolation kit purifies RNA with high RIN scores enhancing suitability for downstream applications.

Flexible and Scalable
Combining RNAdvance Blood chemistry with our Biomek Workstation offers high throughput and minimal hands-on solutions: two 96-well plates of samples can be purified in approximately 2 hours and 15 minutes.

Product Specifications

Application Uses RNA Isolation, RNA Extraction, RNA Sequencing, Nucleic acid sample prep
Format Liquid
Starting Sample Material Cultured Cells
Automated Available Yes
Item Specifications Referenced A47943

Citations

Hore, T. A., von Meyenn, F., Ravichandran, M., Bachman, M., Ficz, G., Oxley, D., . . . Reik, W. (2016, October 25). Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. PNAS, 113(43), 12202-1207. doi:10.1073/pnas.1608679113

  • Used to extract RNA from ESC and EpiSC cell lines

Mesnage, R., Phedonos, A., Biserni, M., Arno, M., Balu, S., Corton, J. C., . . . Antoniou, M. N. (2017, October). Evaluation of estrogen receptor alpha activation by glyphosate-based herbicide constituents. Food and Chemical Toxicology, 108, 30-42. doi:10.1016/j.fct.2017.07.025

  • Used to extract RNA from MCF-7 cells

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Documentation and Related Materials

Title Description
{279C6CA2-9797-4CFE-9CAA-5EAFAC29B60D}
{7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}
{7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}
{7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}
{A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}
{7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
{7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}


Technical Documents