Purification des produits de séquence
Système de purification des produits de séquençage (élimination des colorants) Le kit Agencourt® CleanSEQ® est une méthode reposant sur la technologie SPRI®, technologie unique à billes magnétiques, destinée à purifier les produits de séquençage après une réaction de séquençage du cycle. Le protocole ne comporte que 3 étapes, ne nécessite ni centrifugation, ni filtration et donne des rendements et une qualité de séquence comparativement extraordinaires. Le système Agencourt® CleanSEQ® apporte à l'utilisateur une méthode de purification extrêmement flexible, simple, facilitant l'automatisation et économique.
Choose a Size Selection Model
SPRIselect Left Workflow
SPRIselect Right Workflow
Product Features
SPRIselect chemistry speeds and simplifies nucleic acid size selection for fragment library preparation for next-generation sequencing.
SPRIselect allows tunable size selection from 150 to 800 base pairs to adjust to specific workflow needs.
SPRIselect yields consistent size selections without bead calibration between reagent lots.
Suitable for manual or automated workflows - the SPRIselect method can be run on a variety of Biomek platforms that offer minimal hands-on time.
Product Specifications
| Application Uses | Purification & Clean-up, Nucleic acid sample prep, DNA Size Selection, RNA Size Selection, PCR Clean-up, NGS Clean-up, PCR clean-up |
| Format | Liquid |
| Starting Sample Material | Nucleic Acid |
| Automated Available | Yes |
| Item Specifications Referenced | B23318 |
Citations
Greenwald, W. W., Li, H., Benaglio, P., Jakubosky, D., Matsui, H., Schmitt, A., . . . Frazer, K. A. (2019, March 5). Subtle changes in chromatin loop contact propensity are associated with differential gene regulation and expression. Nature Communications, 10(1054), 1-17. doi:https://doi.org/10.1038/s41467-019-08940-5
- Size Selection of Libraries
Kubo, M., Nishiyama, T., Tamada, Y., Sano, R., Ishikawa, M., Murata, T., . . . Hasebe, M. (2019). Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation. Nucleic Acids Research, 47(9), 4539–4553. doi:10.1093/nar/gkz181
- Used on cDNA Libraries
Behera, V., Stonestrom, A. J., Hamagami, N., Hsiung, C. C., Keller, C. A., Giardine, B., . . . Blobel, G. A. (2019, April 9). Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription. Cell Reports, 27, 400–415. doi:10.1016/j.celrep.2019.03.057
- Used on ChIP-seq Libraries
Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.